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Analytical Chemistry

X-ray

Definition and meaning of X-ray in chemistry.

An X-ray is a form of high-energy electromagnetic radiation with a wavelength ranging from about 0.01 to 10 nanometers. In chemistry, X-rays are widely used to determine the atomic and molecular structure of crystals and to identify elemental composition.

In more detail

Because X-ray wavelengths are on the same order of magnitude as the spacing between atoms in a crystal lattice, they undergo diffraction when passing through crystalline materials. This phenomenon, known as X-ray crystallography, allows chemists to map the electron density of a sample and deduce the precise three-dimensional arrangement of atoms in molecules, ranging from simple salts to complex proteins. Additionally, techniques like X-ray fluorescence are used to determine the elemental composition of materials by analyzing the secondary X-rays emitted when a sample is bombarded with high-energy radiation.

Key facts

FieldAnalytical Chemistry
Wavelength Range0.01 to 10 nm
Key ApplicationX-ray crystallography
Example

Rosalind Franklin used X-ray diffraction to capture an image known as Photo 51, which provided crucial evidence for the double-helix structure of DNA.

Frequently asked questions

How does X-ray crystallography work?

It works by shining a beam of X-rays at a crystal, which scatters the rays into a specific diffraction pattern that can be analyzed to determine the exact arrangement of atoms.