Clear, accurate chemistry definitions 1,227 terms 6 topics 118-element periodic table
General Chemistry

Substance

Definition and meaning of Substance in chemistry.

A substance is a material with definite, fixed composition and distinct physical and chemical properties that remain constant regardless of the sample size. Every sample of a pure substance contains the same elements in identical proportions.

In more detail

Substances differ fundamentally from mixtures because they have uniform composition throughout. A substance can be either an element (like pure oxygen or iron) or a compound (like water or table salt) composed of two or more elements in fixed ratios. The properties of a substance are characteristic and reproducible: water always melts at 0°C and boils at 100°C at standard atmospheric pressure. Once identified, a substance's behavior in specific conditions can be reliably predicted.

Key facts

FieldGeneral Chemistry
CompositionFixed and definite throughout; same elements in same proportions
TypesElements or compounds
Key CharacteristicConstant, reproducible physical and chemical properties
Example

Water (H2O) is a substance because every sample, whether from a glacier or a tap, contains hydrogen and oxygen in a fixed 2:1 atomic ratio (about 11% hydrogen and 89% oxygen by mass) and exhibits identical physical properties (melting point 0°C, boiling point 100°C at 1 atm, density 1.00 g/mL at 4°C).

Frequently asked questions

How does a substance differ from a mixture?

A substance has a fixed composition and specific properties throughout, while a mixture has variable composition and can be separated by physical means without changing the chemical identity of its components.

Can a substance be made of multiple elements?

Yes. Compounds are substances made of two or more elements chemically bonded in fixed proportions, such as sodium chloride (NaCl) and glucose (C6H12O6).

Related terms